Biocompatible chia polysaccharide-chitosan hydrogel and encapsulation of carnitine

9ª Edición

Biocompatible chia polysaccharide-chitosan hydrogel and encapsulation of carnitine

Educación

In our project we use bio-materials to encapsulate carnitine in chia-chitosan beads. The use of the bio-materials minimizes the side effects so bio-compatible structure will be obtained. After the bead is taken to the body it will stay in steady-state un
Our goal in this project is to create a hydrogel with natural materials. Today we see many people with diseases like obesity, diabetes, cardiovascular diseases. Obesity especially has strong relations with fat metabolism. At that point we chose carnitin to be encapsulated in our hydro-gels. With this alternative carrier material the side effects of carnitine can be reduced. And our project is ecofriendly. We wanted to prepare our hydrogel crosslinked with chia polysaccharide and we start with chia seeds. Chia seed has high content of fiber, fat and carbohydrate which is good for our health.We grind the seeds and dry them over the night. After having dry seeds we do a Soxhlet extraction with acetone to deft the grinded seeds. Soxhlet extractor is composed of 4 main parts. We only use the materials left inside the filters. In the end we obtain chia carbohydrate and protein. Next step is finding the best buffer to combine with the grinded chia seed carbohydrate and protein. The options are distilled water, acetate buffer, phosphate buffer and TrisHCl buffer. The main purpose of the buffers is having a stable pH in the solution during the experiment. Different ratios of these buffers will be tried and the one that gives the best results will be used for next steps. To determine the buffer and the ratio preparation of hydrogels will start. We will mix buffers and 1 gr grinded defatted chia seed and wait for 30 minutes in room temperature. This time will let chia to form a mucilage. Then we will centrifuge the mixture and two parts will be formed. We only use the supernatant for gel preparation. All these are for determining the right buffer and the right ratio. After determining the buffer we will determine the amount of chitosan that will form a stable bead but will also release the substance inside. Too much chitosan wouldn’t release the substance inside and too little chitosan wouldn’t encapsulate the substance and form a stable bead for a necessary time. In order to observe the formation of the beads with just chitosan we will drop different amounts of chitosan into NaOH followed by a swelling test. After setting the ratio of chitosan, it’s time to determine the amount of carnitine. Again like chitosan, the amount of carnitine is very important for a stable bead. Different ratios of carnitine will be tried. Supernatant of grinded chia seeds mixed with the buffer, chitosan and various amounts of carnitine will be mixed together and will be put in a NaOH solution for the formation of beads. We will observe the maximum amount of carnitine that will form a stable bead. Our goal is to use the minimum amount of chitosan and maximum amount of carnitine. Finally, after determining the ratio of everything a release study will be conducted. In this study we will monitor the amount of carnitine released from the beads in the body after it’s taken.

Tutor

Olgu Abdis

Equipo

Aslı Gülener

Fulya Boybeyi

Centro

St. Joseph Fransiz Lisesi

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